GSM4851910: COLO320DM JQ1 BRD4 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Digestive tract
Cell type
COLO 320DM
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
COLO320-DM cells
cell type
COLO320-DM
chip antibody
BRD4 (Bethyl Laboratories A301-985A100)
treatment
500 nM JQ1, 6 hours
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated chromatin from cross-linked cells was immunoprecipitated using H3K27ac (Abcam ab4729) and BRD4 (Bethyl Laboratories A301-985A100) antibodies. Following crosslink reversal, RNAse and Proteinase K treatment, immunoprecipitated DNA was purified using the ChIP DNA Clean & Concentrator kit (Zymo Research D5205). Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit.