Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Digestive tract
Cell type
COLO 320DM
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
COLO320-DM cells
cell type
COLO320-DM
chip antibody
BRD4 (Bethyl Laboratories A301-985A100)
treatment
500 nM JQ1, 6 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated chromatin from cross-linked cells was immunoprecipitated using H3K27ac (Abcam ab4729) and BRD4 (Bethyl Laboratories A301-985A100) antibodies. Following crosslink reversal, RNAse and Proteinase K treatment, immunoprecipitated DNA was purified using the ChIP DNA Clean & Concentrator kit (Zymo Research D5205). Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
37766919
Reads aligned (%)
94.2
Duplicates removed (%)
15.4
Number of peaks
2232 (qval < 1E-05)

hg19

Number of total reads
37766919
Reads aligned (%)
92.9
Duplicates removed (%)
15.9
Number of peaks
825 (qval < 1E-05)

Base call quality data from DBCLS SRA